Potato Glucokinase and Fructokinase

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منابع مشابه

Molecular genetics of the human glucokinase regulator-fructokinase (GCKR-KHK) region of chromosome 2p23.

19 Veiga-da-Cunha, M., Courtois, S., Michel, A., Gosselain, E. and Van Schaftingen, E. (1996) J. Biol. Chem. 271,6292-6297 20 Charles, R. St., Harrison, R. W., Bell, G. I., Pilkis, S. J. and Weber, I. T. (1994) Diabetes 43, 784-791 21 Bennett, W. S. and Steitz, T. A. (1978) Proc. Natl. Acad. Sci. U.S.A. 75,4848-4852 22 Gidh-Jain, M., Takeda, J., Xu, L. Z., Lange, A. J., Vionnet, N., Stoffel, M....

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Purification and Properties of Fructokinase from Developing Tubers of Potato (Solanum tuberosum L.).

Fructokinase has been purified from developing potato (Solanum tuberosum L.) tubers by a combination of hydrophobic interaction, affinity chromatography, and gel filtration. The protein has a native molecular mass of approximately 70 kD but is apparently a dimer. Ion-exchange chromatography and two-dimensional western blots resolved three major fructokinases, designated FK-I, FK-II, and FK-III ...

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Modulation of fructokinase activity of potato (Solanum tuberosum) results in substantial shifts in tuber metabolism.

Potato plants (Solanum tuberosum L. cvs Desiree and Record) transformed with sense and antisense constructs of a cDNA encoding the potato fructokinase StFK1 exhibited altered transcription of this gene, altered amount of protein and altered enzyme activities. Measurement of the maximal catalytic activity of fructokinase revealed a 2-fold variation in leaf (from 90 to 180% of wild type activity)...

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Primary structure and characterization of a cDNA clone of fructokinase from potato (Solanum tuberosum L. cv record).

The major source of carbon for starch synthesis in reserve tissues such as the potato (Solanum tuberosum L.) tuber is SUC. In the developing tuber, Suc is hydrolyzed principally by Suc synthase (EC 2.4.1.13) to UDP-Glc and Fru (Preiss, 1982). Fructokinase (EC 2.7.1.4) is believed to catalyze the phosphorylation of the Fru released to yield Fru-6-P. This sugar phosphate can then be used to suppo...

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Bovine liver fructokinase: purification and kinetic properties.

Fructokinase from beef liver has been purified 2300-fold by acid and heat treatment, ammonium sulfate fractionation, and chromatography on Sephadex G-100, DEAE- and CM-cellulose. The purified enzyme is homogeneous by all criteria examined, has a molecular weight of 56 000, and is a dimer of equal molecular weight subunits. The isoelectric point is 5.7. The Michaelis constant for activation by K...

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ژورنال

عنوان ژورنال: Journal of the agricultural chemical society of Japan

سال: 1960

ISSN: 0002-1407,1883-6844

DOI: 10.1271/nogeikagaku1924.34.4_303